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ca9 deactivating peptide  (Novus Biologicals)


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    Novus Biologicals ca9 deactivating peptide
    Schematic representation of the SERS nanoparticles developed herein. The Raman dyes were 4,4′-dipyridyl (s420, red dye), d8-4,4′-dipyridyl (s421, blue dye), and trans -1,2-bis(4-pyridyl)-ethylene (s440, green dye). The blue IgG4 nanoparticle is used as a negative experimental control for active binding of <t>CA9-</t> and CD47-targeted SERS nanoparticles.
    Ca9 Deactivating Peptide, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca9+deactivating+peptide/pmc06202635-162-4-8?v=Novus+Biologicals
    Average 90 stars, based on 2 article reviews
    ca9 deactivating peptide - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype"

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    Journal: ACS Nano

    doi: 10.1021/acsnano.8b03217

    Schematic representation of the SERS nanoparticles developed herein. The Raman dyes were 4,4′-dipyridyl (s420, red dye), d8-4,4′-dipyridyl (s421, blue dye), and trans -1,2-bis(4-pyridyl)-ethylene (s440, green dye). The blue IgG4 nanoparticle is used as a negative experimental control for active binding of CA9- and CD47-targeted SERS nanoparticles.
    Figure Legend Snippet: Schematic representation of the SERS nanoparticles developed herein. The Raman dyes were 4,4′-dipyridyl (s420, red dye), d8-4,4′-dipyridyl (s421, blue dye), and trans -1,2-bis(4-pyridyl)-ethylene (s440, green dye). The blue IgG4 nanoparticle is used as a negative experimental control for active binding of CA9- and CD47-targeted SERS nanoparticles.

    Techniques Used: Control, Binding Assay

    Validation of active targeting using flow cytometry. (a) Flow cytometry histogram showing binding of CD47-targeted SERS nanoparticles to cells with three negative controls. (b) Experiments from (a) were reported in triplicate and plotted in a bar graph. (c) Flow cytometry histogram showing binding of CA9-targeted SERS nanoparticles to cells with three negative controls. (d) Experiments from (c) were conducted in triplicate and plotted in a bar graph.
    Figure Legend Snippet: Validation of active targeting using flow cytometry. (a) Flow cytometry histogram showing binding of CD47-targeted SERS nanoparticles to cells with three negative controls. (b) Experiments from (a) were reported in triplicate and plotted in a bar graph. (c) Flow cytometry histogram showing binding of CA9-targeted SERS nanoparticles to cells with three negative controls. (d) Experiments from (c) were conducted in triplicate and plotted in a bar graph.

    Techniques Used: Flow Cytometry, Binding Assay

    Multiplexed imaging of CD47 and CA9 on cells in suspension. (a) Photograph of cells positive and negative for CD47 and CA9. The top right sample in the 2 × 2 grid is positive for CA9 but negative for CD47. (b) Spectral unmixing of background, s420-CA9, s421-IgG4, s440-CD47. The thick red line represents the fit, and the underlying thick dark blue line is the raw data. Thin lines represent the SERS and background components of the signal. (c) Overlay of the s440, s421, and s420 channel images on the photograph. (d) Calculated CD47:IgG4 and CA9:IgG4 ratio images overlaid on the photograph.
    Figure Legend Snippet: Multiplexed imaging of CD47 and CA9 on cells in suspension. (a) Photograph of cells positive and negative for CD47 and CA9. The top right sample in the 2 × 2 grid is positive for CA9 but negative for CD47. (b) Spectral unmixing of background, s420-CA9, s421-IgG4, s440-CD47. The thick red line represents the fit, and the underlying thick dark blue line is the raw data. Thin lines represent the SERS and background components of the signal. (c) Overlay of the s440, s421, and s420 channel images on the photograph. (d) Calculated CD47:IgG4 and CA9:IgG4 ratio images overlaid on the photograph.

    Techniques Used: Imaging, Suspension

    Approach 1: Tissue classification based on raw, non-normalized SERS signal intensity. (a) Photograph and overlays of unmixed s420-CA9, s421-IgG4, and s440-CD47 images. (b) Stem plot of the non-normalized signal in each channel for all N = 15 samples. (c) Statistical comparison of the binding levels of s421-IgG4 in normal and tumor samples. (d) ROC plot for tissue classification using either all three channels (3-plex) or only the s421-IgG4 channel (passive). (e) Histological analysis of the data point indicated by a black asterisk in frame (b). Blue is DAPI nuclear stain, and red is a CA9 stain.
    Figure Legend Snippet: Approach 1: Tissue classification based on raw, non-normalized SERS signal intensity. (a) Photograph and overlays of unmixed s420-CA9, s421-IgG4, and s440-CD47 images. (b) Stem plot of the non-normalized signal in each channel for all N = 15 samples. (c) Statistical comparison of the binding levels of s421-IgG4 in normal and tumor samples. (d) ROC plot for tissue classification using either all three channels (3-plex) or only the s421-IgG4 channel (passive). (e) Histological analysis of the data point indicated by a black asterisk in frame (b). Blue is DAPI nuclear stain, and red is a CA9 stain.

    Techniques Used: Comparison, Binding Assay, Staining

    Approach 2: Tissue classification based on active-to-passive normalized data. (a) Overlay CA9:IgG4 ratio on same tissue as Figure . (b) CD47:IgG4 ratio. (c) scatterplot of CD47:IgG4 and CA9:IgG4 ratios for N = 15 bladder tissue samples. (d) ROC curve for classifying bladder tissue based on 2-plexed and 3-plexed data. (e) SNR simulations of active-to-passive (Approach 2) and active-to-sum (Approach 3) ratios.
    Figure Legend Snippet: Approach 2: Tissue classification based on active-to-passive normalized data. (a) Overlay CA9:IgG4 ratio on same tissue as Figure . (b) CD47:IgG4 ratio. (c) scatterplot of CD47:IgG4 and CA9:IgG4 ratios for N = 15 bladder tissue samples. (d) ROC curve for classifying bladder tissue based on 2-plexed and 3-plexed data. (e) SNR simulations of active-to-passive (Approach 2) and active-to-sum (Approach 3) ratios.

    Techniques Used:

    Approach 3: Classifying bladder tissue with active-to-sum normalized data. (a–c) CA9:sum, IgG4:sum, and CD47:sum overlays for the same tissue samples as in Figure a,b. (d) CD47:2-sum and CA9:2-sum ratios for all N = 15 tissue samples scanned on the endoscope. Because the 2-sum ratios only depend on either the s440-CD47 or s420-CA9 signal, the 2-sum ratios do not reflect correlations between s440-CD47 and s420-CA9 binding and thus fall directly on each axis. (e) CD47:3-sum and CA9:3-sum ratios for all N = 15 tissue samples. (f) ROC curves for classification of bladder tissue samples based on 3-plexed and 2-plexed active-to-sum ratios.
    Figure Legend Snippet: Approach 3: Classifying bladder tissue with active-to-sum normalized data. (a–c) CA9:sum, IgG4:sum, and CD47:sum overlays for the same tissue samples as in Figure a,b. (d) CD47:2-sum and CA9:2-sum ratios for all N = 15 tissue samples scanned on the endoscope. Because the 2-sum ratios only depend on either the s440-CD47 or s420-CA9 signal, the 2-sum ratios do not reflect correlations between s440-CD47 and s420-CA9 binding and thus fall directly on each axis. (e) CD47:3-sum and CA9:3-sum ratios for all N = 15 tissue samples. (f) ROC curves for classification of bladder tissue samples based on 3-plexed and 2-plexed active-to-sum ratios.

    Techniques Used: Binding Assay

    Summary of Signal Processing Approaches <xref ref-type= a " title="Summary of Signal Processing Approachesa" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Summary of Signal Processing Approaches a

    Techniques Used:



    Similar Products

    ca9 deactivating peptide  (Novus Biologicals)
    90
    Novus Biologicals ca9 deactivating peptide
    Schematic representation of the SERS nanoparticles developed herein. The Raman dyes were 4,4′-dipyridyl (s420, red dye), d8-4,4′-dipyridyl (s421, blue dye), and trans -1,2-bis(4-pyridyl)-ethylene (s440, green dye). The blue IgG4 nanoparticle is used as a negative experimental control for active binding of <t>CA9-</t> and CD47-targeted SERS nanoparticles.
    Ca9 Deactivating Peptide, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca9+deactivating+peptide/pmc06202635-162-4-8?v=Novus+Biologicals
    Average 90 stars, based on 1 article reviews
    ca9 deactivating peptide - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic representation of the SERS nanoparticles developed herein. The Raman dyes were 4,4′-dipyridyl (s420, red dye), d8-4,4′-dipyridyl (s421, blue dye), and trans -1,2-bis(4-pyridyl)-ethylene (s440, green dye). The blue IgG4 nanoparticle is used as a negative experimental control for active binding of CA9- and CD47-targeted SERS nanoparticles.

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Schematic representation of the SERS nanoparticles developed herein. The Raman dyes were 4,4′-dipyridyl (s420, red dye), d8-4,4′-dipyridyl (s421, blue dye), and trans -1,2-bis(4-pyridyl)-ethylene (s440, green dye). The blue IgG4 nanoparticle is used as a negative experimental control for active binding of CA9- and CD47-targeted SERS nanoparticles.

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques: Control, Binding Assay

    Validation of active targeting using flow cytometry. (a) Flow cytometry histogram showing binding of CD47-targeted SERS nanoparticles to cells with three negative controls. (b) Experiments from (a) were reported in triplicate and plotted in a bar graph. (c) Flow cytometry histogram showing binding of CA9-targeted SERS nanoparticles to cells with three negative controls. (d) Experiments from (c) were conducted in triplicate and plotted in a bar graph.

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Validation of active targeting using flow cytometry. (a) Flow cytometry histogram showing binding of CD47-targeted SERS nanoparticles to cells with three negative controls. (b) Experiments from (a) were reported in triplicate and plotted in a bar graph. (c) Flow cytometry histogram showing binding of CA9-targeted SERS nanoparticles to cells with three negative controls. (d) Experiments from (c) were conducted in triplicate and plotted in a bar graph.

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques: Flow Cytometry, Binding Assay

    Multiplexed imaging of CD47 and CA9 on cells in suspension. (a) Photograph of cells positive and negative for CD47 and CA9. The top right sample in the 2 × 2 grid is positive for CA9 but negative for CD47. (b) Spectral unmixing of background, s420-CA9, s421-IgG4, s440-CD47. The thick red line represents the fit, and the underlying thick dark blue line is the raw data. Thin lines represent the SERS and background components of the signal. (c) Overlay of the s440, s421, and s420 channel images on the photograph. (d) Calculated CD47:IgG4 and CA9:IgG4 ratio images overlaid on the photograph.

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Multiplexed imaging of CD47 and CA9 on cells in suspension. (a) Photograph of cells positive and negative for CD47 and CA9. The top right sample in the 2 × 2 grid is positive for CA9 but negative for CD47. (b) Spectral unmixing of background, s420-CA9, s421-IgG4, s440-CD47. The thick red line represents the fit, and the underlying thick dark blue line is the raw data. Thin lines represent the SERS and background components of the signal. (c) Overlay of the s440, s421, and s420 channel images on the photograph. (d) Calculated CD47:IgG4 and CA9:IgG4 ratio images overlaid on the photograph.

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques: Imaging, Suspension

    Approach 1: Tissue classification based on raw, non-normalized SERS signal intensity. (a) Photograph and overlays of unmixed s420-CA9, s421-IgG4, and s440-CD47 images. (b) Stem plot of the non-normalized signal in each channel for all N = 15 samples. (c) Statistical comparison of the binding levels of s421-IgG4 in normal and tumor samples. (d) ROC plot for tissue classification using either all three channels (3-plex) or only the s421-IgG4 channel (passive). (e) Histological analysis of the data point indicated by a black asterisk in frame (b). Blue is DAPI nuclear stain, and red is a CA9 stain.

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Approach 1: Tissue classification based on raw, non-normalized SERS signal intensity. (a) Photograph and overlays of unmixed s420-CA9, s421-IgG4, and s440-CD47 images. (b) Stem plot of the non-normalized signal in each channel for all N = 15 samples. (c) Statistical comparison of the binding levels of s421-IgG4 in normal and tumor samples. (d) ROC plot for tissue classification using either all three channels (3-plex) or only the s421-IgG4 channel (passive). (e) Histological analysis of the data point indicated by a black asterisk in frame (b). Blue is DAPI nuclear stain, and red is a CA9 stain.

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques: Comparison, Binding Assay, Staining

    Approach 2: Tissue classification based on active-to-passive normalized data. (a) Overlay CA9:IgG4 ratio on same tissue as Figure . (b) CD47:IgG4 ratio. (c) scatterplot of CD47:IgG4 and CA9:IgG4 ratios for N = 15 bladder tissue samples. (d) ROC curve for classifying bladder tissue based on 2-plexed and 3-plexed data. (e) SNR simulations of active-to-passive (Approach 2) and active-to-sum (Approach 3) ratios.

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Approach 2: Tissue classification based on active-to-passive normalized data. (a) Overlay CA9:IgG4 ratio on same tissue as Figure . (b) CD47:IgG4 ratio. (c) scatterplot of CD47:IgG4 and CA9:IgG4 ratios for N = 15 bladder tissue samples. (d) ROC curve for classifying bladder tissue based on 2-plexed and 3-plexed data. (e) SNR simulations of active-to-passive (Approach 2) and active-to-sum (Approach 3) ratios.

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques:

    Approach 3: Classifying bladder tissue with active-to-sum normalized data. (a–c) CA9:sum, IgG4:sum, and CD47:sum overlays for the same tissue samples as in Figure a,b. (d) CD47:2-sum and CA9:2-sum ratios for all N = 15 tissue samples scanned on the endoscope. Because the 2-sum ratios only depend on either the s440-CD47 or s420-CA9 signal, the 2-sum ratios do not reflect correlations between s440-CD47 and s420-CA9 binding and thus fall directly on each axis. (e) CD47:3-sum and CA9:3-sum ratios for all N = 15 tissue samples. (f) ROC curves for classification of bladder tissue samples based on 3-plexed and 2-plexed active-to-sum ratios.

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Approach 3: Classifying bladder tissue with active-to-sum normalized data. (a–c) CA9:sum, IgG4:sum, and CD47:sum overlays for the same tissue samples as in Figure a,b. (d) CD47:2-sum and CA9:2-sum ratios for all N = 15 tissue samples scanned on the endoscope. Because the 2-sum ratios only depend on either the s440-CD47 or s420-CA9 signal, the 2-sum ratios do not reflect correlations between s440-CD47 and s420-CA9 binding and thus fall directly on each axis. (e) CD47:3-sum and CA9:3-sum ratios for all N = 15 tissue samples. (f) ROC curves for classification of bladder tissue samples based on 3-plexed and 2-plexed active-to-sum ratios.

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques: Binding Assay

    Summary of Signal Processing Approaches <xref ref-type= a " width="100%" height="100%">

    Journal: ACS Nano

    Article Title: Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype

    doi: 10.1021/acsnano.8b03217

    Figure Lengend Snippet: Summary of Signal Processing Approaches a

    Article Snippet: For negative controls the CA9 deactivating peptide (NB100-417PEP, Novus Biologicals) and a mouse IgG1 isotype (product no. MAB002, R&D Biosystems) for CD47 were used.

    Techniques: